B-Galactosidase Enzyme Activities Essay Example | Topics and Well Written Essays - 1500 words

B-Galactosidase Enzyme Activities - Essay ExampleThe ideas on the regulation of protein deduction in bacteria have mainly arisen from the studies of Perse Monod on the induced synthesis of B-galactosidase in E.coli. It resulted in the breakthrough of regulatory events to the transcription of DNA and the assemblage of functionally related genes into clusters called operons. The specific mechanisms exist to regulate the levels of grammatical construction in the cell. The regulation may be as a result of transcription, translation or messenger RNA stability. In this experiment, there is the regulation of transcription of bacterial genes that are either inducible or repressible enzyme systems. In induced enzymes, there is the breakdown of complex molecules for example lactose induces synthesis of the proteins B-galactosidase, galactose permease and thiogalactoside transacetylase in E.coli. The b-galactosidase enzyme frame in E.coli is an example of an inducible enzyme. It catalyzes the hydrolysis of a wide variety of B-galactosides such as lactose into its constituent substances on that point was of sharing of the reagents labeled control and lactose (L) flask. It was vital that the lactose does not enter the control bottle. Separate, a labeled pipette was reserved to make an addition to, or to sample from the flask. Time was an important doer and lactose was timed at zero time, ninety minutes, hundred and twenty and one hundred and lxxx minutes up to two hundred and forty minutes. From there, sampling procedure included. At the appropriate time, 3ml aliquots were distant from both control(C), and lactose (L) culture and further 0.5mL aliquots from lactose (L) culture placed in a pliable centrifuge tube. Dilution of 0.5mL aliquot one in five by adding 2ml of culture medium was done and labeled the cell L/5. (Michael & Nelson. 2008)